Institute for Genetics

Mouse Genetics and Inflammation

Laboratory

Practical course WS 20011/2012:

Masters Degree (M.Sc.) Module 4: Modern Mouse Genetics

Part II: "Mouse Genetics as a tool to investigate signalling cascades in vivo"

starts on Friday, October 28, 2011

Course overview:

Theoretical part of the module:

28/10/- 04/11/2011

The course will start with a week of theoretical work. You will get a brief introduction to NF-kB signalling and you will be given original research papers describing key findings on the function and regulation of the NF-kB pathway. You will spend this first week with reading of the research papers, literature searches to obtain background information, and in depth discussion of the manuscripts with the course supervisors, who will advise you on how to assemble a ‘journal club’ presentation. At the end of the first week all participants in the course will present their papers in a ‘journal club’, which is part of the course evaluation process.

04/11/2011 Journal club presentations

Course Introduction:

02/11/2011, 10:00, Institut für Genetik, Hörsaal EG

CourseIntroduction (PDF)

Practical part of the module:

07/11/- 18/11/2011

Lectures during the practical part of the module :

07/11/- 11/11/2011

1. Introduction: Immune System
2. Innate Immunity
3. TNF signalling
4. TLR signalling
5. Inflammatory Bowel Disease

14/11/- 18/11/2011

NF-kB in inflammatory diseases: Lessons from mouse models

1. NF-kB mediated transcriptional regulation
2. Inflammatory Liver Disease
3. Inflammatory Skin Disease
4. Atherosclerosis
5. Reports

Protocol due: 02.12.2011

Exam: 24.02.2012

Practical Course instructors:

• Teresa Corona
• Nikos Oikonomou
• Apostolis Polykratis
• Claudia Uthoff-Hachenberg
• Katerina Vlantis
• Patrick Welz

Literature for the theoretical part:

Constitutive IKK2 activation in intestinal epithelial cells induces intestinal tumors in mice. Vlantis K, Wullaert A, Sasaki Y, Schmidt-Supprian M, Rajewsky K, Roskams T, Pasparakis M. J Clin Invest. 2011 Jul 1;121(7).download pdf

NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Pikarsky E, Porat RM, Stein I, Abramovitch R, Amit S, Kasem S, Gutkovich-Pyest E, Urieli-Shoval S, Galun E, Ben-Neriah Y (2004). Nature 431(7007):461-6.download pdf

The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover. Chang L, Kamata H, Solinas G, Luo JL, Maeda S, Venuprasad K, Liu YC, Karin M (2006). Cell 124(3):601-13.download pdf

Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-kappa B. Beg AA, Sha WC, Bronson RT, Ghosh S, Baltimore D (1995). Nature 376(6536):167-70.download pdf

Targeted mutation of TNF receptor I rescues the RelA-deficient mouse and reveals a critical role for NF-kappa B in leukocyte recruitment. Alcamo E, Mizgerd JP, Horwitz BH, Bronson R, Beg AA, Scott M, Doerschuk CM, Hynes RO, Baltimore D (2001). J Immunol. 167(3):1592-600.download pdf

Failure to regulate TNF-induced NF-kappaB and cell death responses in A20-deficient mice. Lee, E. G., D. L. Boone, S. Chai, S. L. Libby, M. Chien, J. P. Lodolce and A. Ma (2000). Science 289(5488): 2350-4. download pdf

De-ubiquitination and ubiquitin ligase domains of A20 downregulate NF-kappaB signalling. Wertz IE, O'Rourke KM, Zhou H, Eby M, Aravind L, Seshagiri S, Wu P, Wiesmann C, Baker R, Boone DL, Ma A, Koonin EV, Dixit VM (2004). Nature 430(7000):694-9.download pdf

Essential function for the kinase TAK1 in innate and adaptive immune responses Sato S, Sanjo H, Takeda K, Ninomiya-Tsuji J, Yamamoto M, Kawai1 T, Matsumoto K, Takeuchi1 O & Akira S (2005). Nat. Immunol. 6(11):1087-1095 download pdf

FADD prevents RIP3-mediated epithelial cell necrosis and chronic intestinal inflammation. Welz PS, Wullaert A, Vlantis K, Kondylis V, Fernández-Majada V, Ermolaeva M, Kirsch P, Sterner-Kock A, van Loo G, Pasparakis M. Nature. 2011 Jul 31;477(7364):330-4. download pdf

Links concerning the practical course:

• Manual

Results Practical:

Practical course WS 2010/2011:

Bachelors Degree (B.Sc.) Teil 1 Molekulares Klonieren

Course Material:

• Manual

Please check again for more information!

Last update 21/10/11