mouse_tail, Illustration of mouse's tail that transforms into a strand of DNA, Credit to Darryl Leja, NHGRI December 3, 2002  

Mouse Genetics and Inflammation

Laboratory

 
 

UniTag

28/06/2017


News

Practical Course SS 2017 12.06.-28.07.2017

Masters Degree (M.Sc.), Module MN-B-SM (G1): Mouse Genetics, Cell Death and Inflammation

Introduction to the module and obligatory safety instruction:


7th of  June 2017, 13:00, Institut für Genetik, seminar room 0.46, ground floor. 

Course overview:

During this course you will participate in two practical parts (I +II), a theoretical week with Journal Club, Lectures and a final exam.

12/06/- 23/06/2017 Practical part I:


“Engineering the mouse genome with CRISPR/Cas and site specific recombinases”

26/06/- 30/06/2017 Journal Club Week:

You will get a brief introduction to NF-kB signalling and you will be given original research papers describing key findings on the function and regulation of the NF-kB pathway. You will spend this week with careful critical reading of the research papers, literature searches to obtain background information and in depth discussion of the manuscripts with assigned tutors, who will advise you on how to assemble a 'journal club' presentation. At the end of that week all participants in the course will present their papers in a 'journal club', which is part of the course evaluation process.

19.06.17 Introduction to NF-kB signalling, 09:00, Institut für Genetik, seminar room 0.46, ground floor.

30.06.17 Jounral Club from 9:30 on,  CECAD, ground floor,  EG.037/038.

03/07/- 21/07/2017 Practical part II:

"Mouse Genetics as a tool to investigate signalling cascades in vivo"
 
The practical parts consits of lectures (if not anounced differently: 9:30, Institut für Genetik, seminar room 0.46, ground floor) and practical training in the laboratory.

Lectures:

  1. Mon, 12.06.17 CRISPR/Cas (Laurens)
  2. Tue, 13.06.17 Modern Mouse Genetics I (Prof. Manolis Pasparakis, 9:00)
  3. Wed, 14.06.17 Nuclease assisted Gene Tergeting (Laurens)

 

 

  1. Mon, 19.06.17 Introduction to NF-kB signalling (Prof. Manolis Pasparakis, 9:00)
  2. Tue, 20.06.17 NF-kB mediated transcriptional regulation (Teresa) 

 

Cell Death Pathways

  1. Wed, 21.06.17 Caspases (Prof. Hamid Kashkar)
  2. Thr, 22.06.17 Apoptosis, External and Internal Pathways (Prof. Hamid Kashkar)
  3. Fri, 23.06.17 TNF signalling (Prof. Manolis Pasparakis, 9:00, Lecture Hall 4th floor)
  4. Wed, 28.06.17 Necroptosis (Prof. Manolis Pasparakis, 9:00)

 

Immunity and Inflammation

  1. Mon, 03.07.17 Introduction: Immune System (Prof. Manolis Pasparakis, 9:00)
  2. Tue, 04.07.17 Innate Immunity (Prof. Manolis Pasparakis, 9:00)
  3. Wed, 05.07.17 TLR signalling (Apostolis) 
  4. Thr, 06.07.17 Inflammasomes (Prof. Hamid Kashkar)

 

NF-kB in inflammatory diseases: Lessons from mouse model

  1.  Mon, 10.07.17 Inflammatory Bowel Disease (Marius)
  2.  Tue, 11.07.17 Inflammatory Liver Disease (Vangelis)
  3.  Wed, 12.07.17 Inflammatory Skin Disease (Sneha)
  4.  Thr, 13.07.17 Atherosclerosis (Apostolis)

 

  1. Mon, 17.07.17 Immunohistochemistry
 
Friday, 21.07.17 reports results (from 9:30 on), Institut für Genetik, seminar room 3rd  floor .


Exam: 28.07.17, 10:00-12:00, Institut für Genetik, lecture hall ground floor.

Protocol due: 26.08.17

Practical Course instructors:

  • Teresa Corona, Claudia Uthoff-Hachenberg Laurens Wachmuth together with other members of the Pasparakis Lab
  • ...

Reading List:

Capecchi M.R. Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat Rev Genet. 2005 Jun;6(6):507-12)

Lewandoski M. Conditional control of gene expression in the mouse. (Nat Rev Genet. 2001 Oct;2(10):743-55.)

Oeckinghaus A., Hayden M.S. and Ghosh S. Crosstalk in NF-κB signaling pathways. (Nat Immunol. 2011 Jul 19;12(8):695-708.)

The new frontier of genome engineering with CRISPR-Cas9 GENOME EDITING. J.A. Doudna and E. Charpentier. (Science. 2014 Nov 28;346(6213))

Necroptosis and its role in inflammation. Pasparakis M., Vandenabeele P. (Nature. 2015 Jan 15;517(7534):311-20. Review.)

Literature for the theoretical part: 

RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation.
Lin J, Kumari S, Kim C, Van TM, Wachsmuth L, Polykratis A, Pasparakis M. Nature. 2016 Dec 1;540(7631):124-128.


NEMO Prevents Steatohepatitis and Hepatocellular Carcinoma by Inhibiting RIPK1 Kinase Activity-Mediated Hepatocyte Apoptosis.  Kondylis V, Polykratis A, Ehlken H, Ochoa-Callejero L, Straub BK, Krishna-Subramanian S, Van TM, Curth HM, Heise N, Weih F, Klein U, Schirmacher P, Kelliher M, Pasparakis M., Cancer Cell. 2015 Nov 9;28(5):582-98.

Phosphorylation-Driven Assembly of the RIP1-RIP3 Complex Regulates Programmed Necrosis and Virus-Induced Inflammation. YoungSik Cho, Sreerupa Challa, David Moquin, Ryan Genga, Tathagat Dutta Ray, Melissa Guildford, and Francis Ka-Ming Chan.  Cell 137, 1112–1123, June 12, 2009 ª.

LUBAC-Recruited CYLD and A20 Regulate Gene Activation and Cell Death by Exerting Opposing Effects on Linear Ubiquitin in Signaling Complexes. Draber P, Kupka S, Reichert M, Draberova H, Lafont E, de Miguel D, Spilgies L, Surinova S, Taraborrelli L, Hartwig T et al. Cell Reports 13, 2258–2272, December 15, 2015.

FADD prevents RIP3-mediated epithelial cell necrosis and chronic intestinal inflammation. Welz PS, Wullaert A, Vlantis K, Kondylis V, Fernández-Majada V, Ermolaeva M, Kirsch P, Sterner-Kock A, van Loo G, Pasparakis M. Nature. 2011 Jul 31;477(7364):330-4.

RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis. Dannappel M, Vlantis K, Kumari S, Polykratis A, Kim C, Wachsmuth L, Eftychi C, Lin J, Corona T, Hermance N, Zelic M, Kirsch P, Basic M, Bleich A, Kelliher M, Pasparakis M. Nature. 2014 Sep 4;513(7516):90-4.

Links concerning the practical course:

Results Practical:

Western p-IkBa:

  • Group1
  • Group2
  • Group3
  • Group4
  • Group5
  • Group6
  • Group7
  • Group8

 

GFP p65 MEFs

  • group1
  • group2
  • group3
  • group4
  • group5
  • group6
  • group7
  • group8

 

FACS:

  • group1
  • group2
  • group3
  • group4
  • group5
  • group6
  • group7
  • group8

 

PCRs

  • group1
  • group2
  • group3
  • group4
  • group5
  • group6
  • group7
  • group8

 

Longterm stimulation microscopy

  • groups 1,3,5,7
  • groups 2,4,6,8

H & E

  • group1
  • group2
  • group3
  • group4
  • group5
  • group6
  • group7
  • group8

 

CD3 stainings

  • group1
  • group2
  • group3
  • group4
  • group5
  • group6
  • group7
  • group8

Please check again for more information!

Last update 31/05/17