Börries Kemper
retired since Nov. 1st, 2003
Institut für Genetik
Zülpicher Str.47
D-50674 Cologne
Tel: 0221-470-5287
Fax: 0221-470-5172
B.Kemper



Research Description:

We are interested in DNA repair including genetic recombination. We isolate and characterize DNA safeguarding enzymes from Prokaryotes and Eukaryotes and study their reactions with target molecules. DNA-branching is a natural event occurring during homologous genetic recombination, and four-armed X-junctions (Holliday-structures) or three-armed Y-junctions comprise two of the most common and transient intermediates. Branched molecules require precise resolution by a nuclease and repair by helper proteins timely before progeny cells devide or viruses package DNA into their newly synthesized heads. DNA-debranching enzymes (X-solvases)like Holliday-structure resolving endonuclease VII (endo VII) from bacteriophage T4 and mitochondrial cruciform cutting endonuclease 1 (CCE1) from yeast S. cerevisiae are among our favorite enzymes. 

 

Group Members

An article from "Kölner Universitäts-Journal" in German.

 

References 1969 - 2003



Kemper,B. (1970). Increased recombinant frequencies with an amber mutation in the gal-operon of E. coli. Mol. Gen. Genet. 107, 107-113.

Kemper,B. and Hurwitz,J. (1973). Studies on T4-induced nucleases. Isolation and characterization of a manganese-activated T4-induced endonuclease. J. Biol. Chem. 248, 91-99.

Kemper,B. and Jaehrig,L. (1974). Suppression of DNA arrest mutants in bacteriophage T4. J. Virol. 13, 1408-1411.

Kemper,B. and Janz,E. (1976). Function of gene 49 of bacteriophage T4. I. Isolation and biochemical characterization of very fast-sedimenting DNA. J. Virol. 18, 992-999.

Kemper,B. and Garabett,M. (1981). Studies on T4-head maturation. 1. Purification and characterization of gene-49-controlled endonuclease. Eur. J. Biochem. 115, 123-131.

Kemper,B., Garabett,M., and Courage,U. (1981). Studies on T4-head maturation. 2. Substrate specificity of gene- 49-controlled endonuclease. Eur. J. Biochem. 115, 133-141.

Kemper,B., Garabett,M., and Courage,U. (1981). Studies on the function of gene 49 controlled endonuclease of phage T4 (endonuclease VII). In Bacteriophage morphogenesis. Proceedings of the 7th biennial Conference on Bacteriophage Morphogensis, M.S.Du Bow, ed. (New York: A.R. Liss Inc.), pp. 151-166.

Courage-Tebbe,U. and Kemper,B. (1982). Construction of gapped circular DNA from phage M13 by in vitro hybridization. Biochim. Biophys. Acta 697, 1-5.

Kemper,B. (1983). Gen-Isolierung aus hoeheren Zellen. Chemie in unserer Zeit 17, 1-9.

Kemper,B., Jensch,F., von Depka-Prondzynski,M., Fritz,H.J., Borgmeyer,U.M., and Mizuuchi,K. (1984). Resolution of Holliday structures by endonuclease VII as observed in interactions with cruciform DNA. Cold Spring Habor Symp. Quant. Biol. 49, 815-825.

Jensch,F. and Kemper,B. (1986). Endonuclease VII resolves Y-junctions in branched DNA in vitro. EMBO J. 5, 181-189.

Kleff, S. and Kemper, B. Repair of heteroduplex-loops is initiated by endonuclease VII in vitro. Journal of Cellular Biochemistry Sup 12A/E136. 1988.

Kleff,S. and Kemper,B. (1988). Initiation of heteroduplex-loop repair by T4-encoded endonuclease VII in vitro. EMBO J. 7, 1527-1535.

Kemper,B., Pottmeyer,S., Solaro,P., and Kosak,H. (1990). Resolution of DNA-secondary structures by endonuclease VII (Endo VII) from phage T4. In Structure & Methods Vol.1: Human Genome Initiative & DNA Recombination, R.H.Sarma and M.H.Sarma, eds. (Schenectady, N.Y.: Adenine Press), pp. 215-229.

Kosak,H.G. and Kemper,B. (1990). Large-scale preparation of T4 endonuclease VII from over- expressing bacteria. Eur. J. Biochem. 194, 779-784.

Kemper,B. (1991). Die dynamische DNA. Forschung in Köln 20-28.

Kemper,B. (1992). Resolution of Recombination Intermediates by Endonuclease VII in vitro. In Molecular Biology of the Cell, W.Doerfler, ed. (Weinheim: VCH Verlagsgesellschaft mbH), pp. 82-96.

Pottmeyer,S. and Kemper,B. (1992). T4 endonuclease VII resolves cruciform DNA with nick and counter-nick and its activity is directed by local nucleotide sequence. J. Mol. Biol. 223, 607-615.

Solaro, P., Birkenkamp, K., Pfeiffer, P., and Kemper, B. Holliday-structures resolving T4 endonuclease VII initiates the correction of mismatched bases through the combined action of T4 DNA-polymerase and T4-ligase in synthetic oligonucleotides in vitro. Journal of Cellular Biochemistry Sup 16B, 97/F634. 1992.

Flemming,M., Deumling,B., and Kemper,B. (1993). Function of gene 49 of bacteriophage T4 III. Isolation of Holliday-structures from very fast-sedimenting DNA. Virology 196, 910-913.

Kemper,B., Solaro,P., and Birkenkamp,K. (1993). Endonuclease VII of phage T4 can initiate mismatch repair in vitro. J. Cell. Biochem. Suppl. 17C, abstr.-165.

Solaro,P.C., Birkenkamp,K., Pfeiffer,P., and Kemper,B. (1993). Endonuclease VII of phage T4 triggers mismatch correction in vitro. J. Mol. Biol. 230, 868-877.

Birkenkamp,K. and Kemper,B. (1995). In vitro processing of heteroduplex loops and mismatches by endonuclease VII. DNA Res. 2, 9-14.

Golz,S., Birkenbihl,R., and Kemper,B. (1995). Improved large scale preparation of phage T4 endonuclease VII overexpressed in E. coli. DNA Res. 2, 277-284.

Kemper, B. and Birkenkamp, K. Strand transfer protein gpuvsX mediates strand exchange across asymmetric inhomologies (loops) in short oligonucleotides in vitro. Journal of Cellular Biochemistry Supplement 21A, 321-Abstract C5-421. 1995.

Solaro,P., Greger,B., and Kemper,B. (1995). Detection and partial purification of a cruciform-resolving activity (X-solvase) from nuclear extracts of mouse B-cells. Eur. J. Biochem. 230, 926-933.

Birkenkamp,K. and Kemper,B. (1996). Bacteriophage T4 strand transfer protein UvsX tolerates symmetric and asymmetric heterologies in short double-stranded oligonucleotides. J. Mol. Biol. 259, 622-631.

Kupfer,C. and Kemper,B. (1996). Reactions of mitochondrial cruciform cutting endonuclease 1 (CCE1) of yeast Saccharomyces cerevisiae with branched DNAs in vitro. Eur. J. Biochem. 238, 77-87.

Birkenkamp-Demtroeder,K., Golz,S., and Kemper,B. (1997). Inhibition of Holliday structure resolving Endonuclease VII of bacteriophage T4 by recombination enzymes UvsX and UvsY. J. Mol. Biol. 267, 150-162.

Golz,S., Christoph,A., Birkenkamp-Demtroeder,K., and Kemper,B. (1997). Identification of amino acids of endonuclease VII essential for binding and cleavage of cruciform DNA. Eur. J. Biochem. 245, 573-580.

Kemper, B. and Thiemann, F. Lysyl tRNA synthetase from yeast S. cerevisiae binds to cruciform DNA via single-stranded loops. Keystone Symposium on: Aminoacyl tRNA synthetases in biology & disease . 1997.

Kemper,B. (1997). Branched DNA resolving enzymes (X-solvases). In DNA Damage and Repair. Biochemistry, Genetics and Cell Biology, J.A.Nickoloff and M.Hoekstra, eds. (Totowa: Humana Press), pp. 179-204.

Birkenbihl,R.P. and Kemper,B. (1998). Endonuclease VII has two DNA-binding sites each composed from one N- and one C-terminus provided by different subunits of the protein dimer. EMBO J 17, 4527-4534.

Birkenbihl,R.P. and Kemper,B. (1998). Localization and characterization of the dimerization domain of holliday structure resolving endonuclease VII of phage T4. J Mol Biol 280, 73-83.

Christoph,A., Heesberg,G., and Kemper,B. (1998). Epitope mapping of T4 endonuclease VII with monoclonal antibodies reveals importance of both ends of the protein for target binding. J Mol Biol 277, 529-540.

Golz,S., Birkenkamp-Demtroeder,K., and Kemper,B. (1998). Enzymatic mutation detection. Procedure for screening and mapping of mutations by immobilised endonuclease VII. Nucleic. Acids. Res. 26, 1132-1133.

Golz,S., Greger,B., and Kemper,B. (1998). Enzymatic mutation detection. Phosphate ions increase incision efficiency of endonuclease VII at a variety of damage sites in DNA. Mutat. Res. 382, 85-92.

Greger,B. and Kemper,B. (1998). An apyrimidinic site kinks DNA and triggers incision by endonuclease VII of phage T4. Nucl. Acids Res. 26, 4432-4438.

Kupfer,C., Lee,S., and Kemper,B. (1998). Binding of endonuclease VII to cruciform DNA. Visualization in the electron microscope. J. Biol. Chem. 273, 31637-31639.

Golz,S. and Kemper,B. (1999). Association of Holliday-structure Resolving Endonuclease VII with gp20 from the Packaging Machine of Phage T4. J Mol Biol 285, 1131-1144.

Golz,S. and Kemper,B. (1999). Enzymatic mutation detection: enrichment of heteroduplexes from hybrid DNA mixtures by cleavage-deficient GST-tagged endonuclease VII. Nucleic Acids Res. 27, e7.

Kemper,B. (1999). Bacteriophages as models for differentiation. In Biology of the prokaryotes, J.W.Lengeler, G.Drews, and H.G.Schlegel, eds. (Stuttgart: Thieme Verlag), pp. 602-623.

Kemper, B., Birkenkamp-Demptröder, K., and Golz, S. Mismatch detection techniques. Variagenics, Inc. Cambridge MA. 243,558[6,110,684]. 29-8-2000. 2-2-1999.

Birkenbihl,R.P., Neef,K., Prangishvili,D., and Kemper,B. (2001). Holliday junction resolving enzymes of archaeal viruses SIRV1 and SIRV2. J. Mol. Biol. 309, 1067-1076.

Hartung,M., Slack,M., and Kemper,B. (2001). Analyses of spontaneous mutations of cloned gene 49 of phage T4. Mutat. Res. 473, 201-210.

Birkenbihl,R.P. and Kemper,B. (2002). High affinity of endonuclease VII for the Holliday structure containing one nick ensures productive resolution. J. Mol. Biol. 321, 21-28.

Neef, K., Birkenbihl, R. P., and Kemper, B. (2002). Holliday Junction Resolving Enzymes from Eight Hyperthermophile Archaea Differ in Reactions with Cruciform DNA. Extremophiles 6, 359-367.

Rass,U. and Kemper,B. (2002). Crp1p, a new cruciform DNA-binding protein in the yeast Saccharomyces cerevisiae. J. Mol. Biol. 323, 685-700.

 

Patents

Cotton, N. R. G., Youil, R., and Kemper, B. Detection of mutation by resolvase cleavage. Avitech Diagnostics, Inc. Malvern PA. 714,626[5,217,863]. 16-12-1997. 19.

Kemper, B., Golz, S., and Birkenbihl, R. P. Overexpression of recombinant bacteriophage T4 endonuclease VII and uses thereof. Variagenics, Inc Cambridge MA. 621,708[5,824,530]. 1998. 28-3-1996.

Kemper, B., Birkenkamp-Demptröder, K., and Golz, S. Mismatch detection techniques. Variagenics, Inc. Cambridge MA. 243,558[6,110,684]. 29-8-2000. 2-2-1999.

B.Kemper, Inst. f. Genetik, Uni Köln 
last change: March 25, 2004